CasNo： 367-93-1
MF： C9H18O5S
Appearance： white crystalline powder
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Isopropyl-beta-D-thiogalactopyranoside is white crystalline powder, while it's Molecular Formula is C9H18O5S. Isopropyl-β-D-thiogalactoside is used as a reagent in molecular biology. It is used as an effective β-galactosidase inducer of protein expression where the gene is under the control of the lac operator. It is used with 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside to identify the non-recombinant plasmid in cloning experiments by blue-white screen methodology.
The CAS number of Isopropyl-beta-D-thiogalactopyranoside is 367-93-1.
More information of Isopropyl-beta-D-thiogalactopyranoside 367-93-1 are:
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Synonyms |
Galactopyranoside,isopropyl 1-thio- (7CI);Galactopyranoside, isopropyl 1-thio-, b-D- (8CI);IPTG;Isopropyl 1-thio-b-D-galactopyranoside;Isopropyl thio-b-D-galactopyranoside;Isopropyl thio-b-D-galactoside;Isopropyl b-thiogalactopyranoside;Isopropyl b-thiogalactoside; |
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CAS Number |
367-93-1 |
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Molecular Formula |
C9H18O5S |
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Molecular Weight |
238.305 |
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Density |
1.37 g/cm3 |
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Melting Point |
105 ºC |
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Boiling Point |
438.4 ºC at 760 mmHg |
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Flash Point |
219 ºC |
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HS CODE |
29389090 |
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PSA |
115.45000 |
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LogP |
-1.07210 |
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Pka |
13.00±0.70(Predicted) |
IPTG (also known as Isopropyl-β-D-Thiogalactopyranoside) is a molecular biology reagent that functions as an inducer of galactosidase activity by binding to and inhibiting the repressor. It is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator. IPTG cannot be hydrolyzed or broken down by the E. coli therefore the concentration remains constant during cell replication. IPTG is often used at a final concentration ranging from 0.5-1.0 mM. X-Gal is often used with IPTG since it provides an easy visual method to monitor whether protein expression has occurred. X-Gal as the name implies contains a galactose group that once metabolized causes a bright blue color change.
InChI:InChI=1/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9?/m1/s1
Articles related to Isopropyl-beta-D-thiogalactopyranoside:
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Article |
Source |
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Effect of Photocaged Isopropyl β-d-1-thiogalactopyranoside Solubility on the Light Responsiveness of LacI-controlled Expression Systems in Different Bacteria |
Hogenkamp, Fabian,Hilgers, Fabienne,Knapp, Andreas,Klaus, Oliver,Bier, Claus,Binder, Dennis,Jaeger, Karl-Erich,Drepper, Thomas,Pietruszka, J?rg , p. 539 - 547 (2021) |
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Photocatalysis Enables Visible-Light Uncaging of Bioactive Molecules in Live Cells |
Wang, Haoyan,Li, Wei-Guang,Zeng, Kaixing,Wu, Yan-Jiao,Zhang, Yixin,Xu, Tian-Le,Chen, Yiyun supporting information, p. 561 - 565 (2019/01/04) |
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